What are OrganaBio's primary human monocytes?

OrganaBio's monocyte product is a cryopreserved primary human monocyte population isolated from adult peripheral blood leukopaks. Monocytes serve as starting material for macrophage differentiation, dendritic cell generation, innate immunity research, and emerging CAR-macrophage (CAR-M) cell therapy development.

How are monocytes isolated to preserve phenotype?

Monocytes are isolated from fresh OrganaBio leukopaks using validated selection methods designed to preserve CD14+ phenotype and differentiation potential. Same-facility collection and isolation minimize the activation and phenotype shift that occur when monocytes are isolated from overnight-shipped leukopaks.

What ships on the CoA with a monocyte order?

Every monocyte order ships with a Certificate of Analysis including CD14+ purity by flow cytometry, post-thaw viability, and donor information. High-resolution HLA typing across six genes is available on the CoA. Custom characterization panels are available at order.

Can monocytes be differentiated into macrophages downstream?

Yes. OrganaBio's monocytes are isolated to preserve differentiation potential for macrophage generation protocols (GM-CSF or M-CSF driven), dendritic cell generation (GM-CSF plus IL-4), and other myeloid lineage differentiation workflows. For programs preferring to skip the differentiation step, pre-differentiated primary human macrophages are also available.

Are disease-state monocytes available?

Yes. Disease-state monocytes are available on request across a range of autoimmune, inflammatory, and oncology indications. Because HemaCenter manages donor recruitment directly, disease-state sourcing is handled in-house rather than through third-party networks.

What applications use primary human monocytes?

Monocytes are used for macrophage differentiation and polarization research, dendritic cell generation for immunotherapy studies, CAR-M cell therapy process development, innate immunity research, inflammatory disease modeling, and cell therapy programs requiring myeloid starting material.

Can I get donor-matched monocytes and macrophages?

Yes. Because OrganaBio isolates both monocytes and macrophages from the same donor pool, donor-matched monocyte and differentiated macrophage material can be supplied from a single donor, which removes the lot-to-lot variance common when the two products come from different suppliers.

CD14+ Monocytes from Peripheral Blood

Name: CD14+ Monocytes cells (Peripheral Blood)
Brand: OrganaBio

CD14+ Monocytes cells (Peripheral Blood)

Cryopreserved human CD14+ monocytes from adult peripheral blood leukopaks. Used for macrophage differentiation, dendritic cell generation, innate immunity research, drug screening, and biomarker discovery. IRB-approved donor screening with documentation on every lot.

Description

Quick summary

Monocytes isolated from peripheral blood leukopaks via CD14+ enrichment. Common starting material for macrophage and dendritic cell differentiation studies. Sourced from OrganaBio’s recallable donor pool with full 14-marker infectious disease screening and high-resolution NGS HLA typing. Available as RUO and cGMP from one quality system. Best fit: macrophage polarization studies, monocyte-derived dendritic cell programs, and innate immunity research.

LeukoPAC-MN-PB – Cryopreserved Human CD14+ Monocytes (Adult Peripheral Blood)

LeukoPAC-MN-PB delivers cryopreserved human CD14+ monocytes isolated from adult peripheral blood leukopaks. CD14+ monocytes are circulating myeloid cells used as starting material for macrophage differentiation, dendritic cell generation, innate immunity studies, drug screening, cancer immunology, and biomarker discovery.

Applications

OrganaBio CD14+ monocytes are used to generate M1 pro-inflammatory or M2 anti-inflammatory macrophages, differentiate immature and mature dendritic cells for vaccine and antigen-presentation work, study phagocytosis and toll-like receptor signaling, evaluate drug candidates targeting monocyte/macrophage biology in inflammation and oncology models, investigate tumor-associated macrophage biology, and profile monocyte surface markers, secretomes, and transcriptomes for biomarker identification.

Donor Program

Cells are isolated from OrganaBio’s recallable donor program of over 50,000 donors. Researchers can request the same donor for repeat orders across multiple product lines, enabling longitudinal studies, process development with consistent starting material, and matched donor panels.

Quality and Documentation

Donors are screened under IRB-approved consent and FDA 21 CFR 1271 donor eligibility criteria. Each lot ships with documentation including donor screening, immunophenotyping by flow cytometry, HLA Class I and II typing, and cryopreservation details. FDA Registered. cGMP available.

Configurations

Available cryopreserved at 5×10⁶, 10×10⁶, and 25×10⁶ cells per vial. Custom vialing, custom donor criteria, and fresh format are available on request. Cells are for preclinical research use only and not for use in humans.

For full specifications, applications, and an interactive donor program overview, see the CD14+ Monocytes product page.

Need custom monocyte isolation or specific donor criteria? Through our CDMO services, we deliver custom isolation (specific subsets, fresh format, disease-state donors) with full quality documentation. Explore Cell Processing Services →

Frequently Asked Questions

How are CD14+ monocytes isolated?

CD14+ monocytes are isolated from peripheral blood leukopaks via immunomagnetic enrichment, yielding a high-purity monocyte population suitable for differentiation studies.

What downstream applications use CD14+ monocytes?

CD14+ monocytes from peripheral blood are common starting material for macrophage polarization studies, monocyte-derived dendritic cell programs, innate immunity research, and inflammation models.

What screening do monocyte donors undergo?

Each donor is screened against 14 infectious disease markers per 21 CFR 1271, plus CMV, EBV, and alloantibodies. NGS HLA typing across HLA-A, B, C, DR, DQ, DP is provided.

Are CD14+ monocytes available at cGMP grade?

Yes. The same donor pool serves both RUO and cGMP grades under one quality system. Programs do not requalify donors when moving research-to-clinical.

Can I get larger volumes or specific donor characteristics?

Yes. Custom volumes, donor characteristics, and HLA-specific selections are available. Contact OrganaBio for quotes on non-standard requests.

Ordering information

Scope LeukoPAC-MN-PB cryopreserved CD14+ monocytes for your program

Use this route when the program needs CD14+ monocytes for macrophage differentiation, dendritic-cell generation, innate-immunity research, or drug screening.

Source and format	Cryopreserved human CD14+ monocytes isolated from adult peripheral-blood leukopaks via CD14+ enrichment.
Published specifications	The current record lists 5×10⁶, 10×10⁶, and 25×10⁶ cells per vial. Donor characterization includes 14-marker screening and NGS HLA typing across HLA-A, B, C, DR, DQ, and DP.
Availability	Product-specific lot status, donor criteria, quantity, grade, documentation, and timing are confirmed with the OrganaBio scientific team.
Documentation	Each lot is described as shipping with donor screening, flow-cytometry immunophenotyping, HLA Class I/II typing, and cryopreservation details.
Storage and shipping	The cryopreserved format requires product-specific storage, shipment, and handling instructions, which are confirmed before shipment.
Intended use and grade	RUO and cGMP are documented from the same donor pool; the product record states that cells are for preclinical research use and not for use in humans.

CD14+ Monocytes cells (Peripheral Blood)

Description